8.3MP-21MP, USB3.2, 400-1000nm CMOS Microscope Camera

| Features |
| 3.3 µm / 2.4µm / 2.9µm multiple pixel size options |
| USB 3.2 Gen 2 interface with 10 Gb/s ultra-high bandwidth |
| Color, Rolling shutter |
| High frame rate capture, up to 240 fps on certain models |
| Selectable 8-bit / 12-bit data output modes |
| Supports hardware and software binning |
| Standard C-mount lens interface |
| CE/FCC Cerifcation,Multi-platform SDK |
The SDK supports development languages such as C/C++, C #/VB.net, Python, Java, etc,
Third-party support including LabVIEW, MATLAB, Micro-Manager, DirectShow, and TWAIN.
| Common Specifications | |
| Model | VIS0321CU3.2-NC, VIS0220CU3.2-NC, VIS028CU3.2-NC |
| Sensor | Sony IMX CMOS sensors |
| Shutter Type | Rolling Shutter |
| Color Type | Color |
| Data Interface | USB3.2 Gen2 (Type-C) |
| Lens Mount | C-mount |
| Binning Mode | Hardware 2×2, 3×3, 4×4; Software 2×2, 3×3, 4×4 |
| Power Supply | USB Type-C interface power |
| Operating Temperature | -10 °C ~ +50 °C |
| Storage Temperature | -30 °C ~ +70 °C |
| Operating Humidity | 20%~80%RH |
| Storage Humidity | 10%~60%RH |
| Software | AttosView / SDK |
| Operating System | Windows/Linux/macOS/Android multi-platform SDK (native C/C++, C#/VB.NET, Python, Java, DirectShow, TWAIN, etc.) |
| Certification | CE, FCC |

| Parameter | Specification |
| Dimension | 68mm×68mm×41.4mm |
| Lens interface | C-mount |

| Item | Specification |
| 1 | USB 3.2 Gen 2 interface |
ascamsdk support a variety of APIs, including: Native C/C++,.NET/C#/VB.NET, Python, Java, DirectShow, Twain, LabView, Matlab, etc. Compared with other APIs, Native C/C++ API as a low-level API is characterized by using pure C/C++ development without relying on other runtime libraries. The interface is simple and the control is flexible.
AttosTek provides extensive third-party software support for its UV-Vis cameras and SWIR cameras, facilitating rapid integration and use in various development environments. This includes SDK and demo programs for LabVIEW and MATLAB, as well as DirectShow drivers, Micromanager, and TWAIN SDK drivers. These features enable seamless compatibility with mainstream development environments and third-party software, greatly simplifying the workflow for system integration and secondary development.
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AttosView Recommended System Requirements |
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Operating System |
Microsoft® Windows® XP / Vista / 7 / 8 /10 /11(32 & 64 bit), Mac OSX, Linux |
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Processor (CPU) |
≥3.0 GHz Intel Core i5 or Higher |
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Memory (RAM) |
≥8 GB |
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Hard Drive |
NVMe Solid State Drive (SSD) |
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Graphics Card |
Dedicated Adapter with ≥256 MB RAM |
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Motherboard |
USB 3.0 (-USB) Cameras: Integrated Intel USB 3.0 Controller or One Unused One Unused PCle3.0*16 Slot |
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Connectivity |
Internet Connectivity for Driver Installation |

Q1: What advantages does this camera have for fluorescence imaging (GFP/RFP/DAPI)?
This series of cameras is suitable for fluorescence imaging (GFP/RFP/DAPI). Its main advantages include:
High sensitivity: Low readout noise (as low as 1.07 e⁻) ensures high signal-to-noise capture of weak fluorescence signals.
Wide spectral compatibility: VIS028CU3.2-NC covers 400–1000 nm, compatible with everything from DAPI (~460 nm) to near-infrared fluorescent dyes.
Fast real-time preview: High frame rates ensure real-time observation of fluorescence signals and quick focusing.
Multi-platform software support: Comes with AttosView software and SDK to meet professional fluorescence imaging acquisition needs.
Q2: How to choose between VIS0321CU3.2-NC, VIS0220CU3.2-NC, and VIS028CU3.2-NC based on the application scenario?
All three cameras are microscope cameras, but they have different pixel sizes and resolutions:
The bigger the pixel, the stronger the light sensitivity. The 2.9 µm (VIS028) and 3.3 µm (VIS0321) pixels perform better in low-light conditions.
If you need to capture fine details, VIS0321CU3.2-NC (21MP, 4/3″ sensor) and VIS0220CU3.2-NC (20MP, 1″ sensor) are recommended. These two cameras have higher resolution and make it easier to see details.
If you need a wider wavelength range, VIS028CU3.2-NC is recommended, as it has a spectral range of 400–1000 nm (the other two are 400–700 nm).
Q2: What's the advantage of this camera's USB 3.2 Gen 2 interface compared to the traditional USB 3.0?
This series of cameras uses a USB 3.2 Gen 2 interface, offering an ultra-high bandwidth of 10 Gb/s, which is 2–3 times faster than traditional USB 3.0 models. This advantage is especially noticeable in high-resolution, high-frame-rate imaging scenarios—for example, the VIS0321CU3.2-NC can reach 240 fps (8 bit) in 1760×1318 ROI mode. The high bandwidth of USB 3.2 Gen 2 ensures that massive amounts of image data are transmitted in real time without any frame drops or delays.
Color Microscope Camera with Sony IMX CMOS Sensor & USB3.2 Interface
| Model | VIS0321CU3.2-NC | VIS0220CU3.2-NC | VIS028CU3.2-NC |
| Sensor Model | Sony IMX472 | Sony IMX383 | Sony IMX585 |
| Spectral Range | 400nm-700nm | 400nm-700nm | 400nm-1000nm |
| Pixel Size | 3.3 µm × 3.3 µm | 2.4µm × 2.4µm | 2.9µm × 2.9µm |
| Sensor Size | 4/3″ | 1″ | 1/1.2″ |
| Frame Rate & Resolution | 8bit: 49fps @5280 x 3956 12bit: 25fps @5280 x 3956 8bit: 115fps@2640 x 1978 12bit: 55fps @2640 x 1978 8bit: 240fps@1760 x 1318 12bit: 117fps@1760 x 1318 |
8bit: 49fps @5472×3648 12bit: 24fps @5472×3648 8bit: 49fps @2736×1824 12bit: 24fps @2736×1824 8bit: 120fps@1824×1216 12bit: 120fps@1824×1216 |
8bit: 88fps@3840 x 2160 12bit: 60fps@3840 x 2160 8bit: 60fps@1920 x 1080 12bit: 60fps@1920 x 1080 |
| Dynamic Range | 72 dB | 71.6dB(HCG); 72.0dB(LCG) | |
| Sensitivity | 982.8 mV (G), 0.55 mV dark current | 411mV | 5970(mV/lux/s); 0.13mV with 1/30s |
| Readout Noise | 4.58 e⁻ | 3.48e⁻ | 1.07e⁻(HCG); 6.69e⁻(LCG) |
| Full well | 24.6ke⁻ | 18.042ke⁻ | 4.061ke⁻(HCG); 39.162ke⁻(LCG) |
| SNRmax | 43.9 dB | 42.6dB | 36.1dB(HCG); 45.9dB(LCG) |
| Conversion Gain | 6.017 e⁻/ADU | 4.410e⁻/ADU | 0.994e⁻/ADU(HCG); 9.582e⁻/ADU(LCG) |
| Data Format | 8/12 bit | 10/12bit | |
VIS028CU3.2-NC
8.3MP Color CMOS Camera with 2.9µm Pixel, USB 3.2 Interface, 88fps
VIS0220CU3.2-NC
20MP Color CMOS Camera with 2.4µm Pixel, USB 3.2 Interface, 49fps
VIS0321CU3.2-NC
21MP Color CMOS Camera with 3.3µm Pixel, USB 3.2 Interface, 49fps
Optional accessories – lenses
AttosView offers an optional YLGD series continuous zoom optical system for visible light cameras, designed specifically for high-precision imaging scenarios. The YLGD series continuous zoom optical system series provides multiple focal length options and is suitable for various professional applications such as security surveillance, industrial vision inspection, and scientific observation, ensuring excellent image clarity, operational flexibility, and environmental adaptability. For inquiries, please contact sales@attostek.com






